| 番茄转ER-sHSP基因植株构建及其抗冷性研究 |
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| 引用本文： | 赵春梅,王丽,伊淑莹,刘箭. 番茄转ER-sHSP基因植株构建及其抗冷性研究[J]. 园艺学报, 2006, 33(5): 989-994 |
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| 作者姓名： | 赵春梅 王丽 伊淑莹 刘箭 |
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| 作者单位： | (山东师范大学生命科学学院, 山东济南250014) |
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| 基金项目： | 国家自然科学基金项目(30270132) |
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| 摘 要： | 将CaMV 35S启动子驱动的内质网小分子热激蛋白基因导入番茄, 比较转基因、未转基因和转空载体番茄的抗冷能力。冷胁迫下, 同对照相比, 转基因番茄的冷害症状轻, 叶绿素含量的损失少, 体内积累的丙二醛(MDA) 含量少, 电解质外渗程度低, 具有较高的净光合速率和最大光化学效率, 并易于恢复由低温所引起的光抑制, 表明转基因番茄具有较强的冷胁迫耐性, 说明内质网小分子热激蛋白在植物抗冷过程中发挥重要作用。
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| 关 键 词： | 番茄 内质网 小分子热激蛋白 转基因 冷胁迫 |
| 文章编号： | 0513-353X（2006）05-0989-06 |
| 收稿时间： | 2006-04-14 |
| 修稿时间： | 2006-04-142006-09-20 |
The Construction and the Chilling-Resistance Ability of Endoplasmic Reticulum Small Heat Shock Protein (ER-sHSP) Transgenic Tomato Plants |
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| Zhao Chunmei,Wang Li,Yi Shuying,Liu Jian. The Construction and the Chilling-Resistance Ability of Endoplasmic Reticulum Small Heat Shock Protein (ER-sHSP) Transgenic Tomato Plants[J]. Acta Horticulturae Sinica, 2006, 33(5): 989-994 |
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| Authors: | Zhao Chunmei Wang Li Yi Shuying Liu Jian |
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| Affiliation: | (College of Life Sciences, Shandong Normal University, Ji'nan, Shandong 250014, China) |
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| Abstract: | The full-length endoplasmic reticulum small heat shock protein ( ER-sHSP) cDNA using CaMV 35S p romoterwas transformed into the genome of tomato plants. The chilling tolerance of transgenic tomato plants, non2transgenic tomato plants and pROKⅡ-transformed tomato plants were studied. Under chilling stress treatment, compared with non-transgenic and pROKⅡ-transformed tomato plants, transgenic tomato plants exhibited slighter cold-injured symptoms, suffered less destruction of chlorophyll and electrolyte leakage. The content of MDA of transgenic tomato plants was lower than that of non-transgenic and pROKⅡ-transformed tomato plants, and transgenic tomato plants could keep up higher value of net photosynthetic rate, Fv/Fm and could recover quickly from chilling-induced photosynthetic inhibition. The results showed that ERsHSP played a key role in enhancing the chilling-resistance ability of plants. |
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| Keywords: | Tomato Endoplasmic reticulum Small heat shock protein Transgenic Chilling stress |
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