Pollen donor impact on progenies of pseudogamous blackberries (Rubus subgen. Rubus) |
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Authors: | Gun Werlemark Hilde Nybom |
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Institution: | 1. Balsg?rd – Department of Crop Sciences, Swedish University of Agricultural Sciences, Fj?lkestadsv?gen 123-1, 291 94, Kristianstad, Sweden
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Abstract: | A series of intra- and interspecific crosses in Rubus was evaluated with DNA markers to assess the proportion of apomictically vs. sexually derived progeny plants. The species
were well separated from each other with these markers and the interspecific hybrids were easy to ascertain. Ten plants each
were derived by selfing of R. hartmanni, R. polyanthemus and R. vestitus, and evaluated with RFLP. Of these plants, only one R. hartmanni plant appeared to be sexually derived, whereas the remainder appeared to have arisen by apomixis. Between 10 and 19 progeny
plants, derived by open pollination of R. Hartmanni, R. infestus, R. laciniatus and R. vestitus, respectively (total of 63plants), were evaluated with RFLP. Of these, 6 plants (approx. 10%) showed as lightly deviating
band pattern, which may indicate sexual seed set but could also result from experimental artefacts. The three species combinations
1) R. hartmanni and R. sprengelii, 2) R. infestus and R. vestitus, and 3)R. laciniatus and R. polyanthemus were cross-pollinated reciprocally and the resulting progenies evaluated with RAPD (combinations 1 and 2)and leaf shape (combination
3). Combination1 produced 20% (3 out of 15 progenies)sexually derived progenies, combination 2produced 100% (26 progenies)
and combination 3 produced 64% (16 out of 26progenies), respectively. The hybrid progenies appeared to have been derived through
pollination of unreduced as well as reduced egg cells. The ability to produce interspecific hybrid progenies is most probably
dependent upon the species combination.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | apomixis RAPD RFLP Rubus |
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