猪Caspase-3基因的克隆及序列分析

天冬氨酸特异性半胱氨酸蛋白酶(C aspase)是与细胞凋亡有关的一类蛋白酶,其中C aspase-3是细胞凋亡的最终执行者。为了进一步研究C aspase-3的生物学功能,从PK-15细胞中提取总RNA,用RT-PCR方法扩增出猪C aspase-3基因,序列分析表明克隆的C aspase-3基因与G enB ank上登录的猪C aspase-3基因核苷酸与推导的氨基酸序列同源性分别为98.9%和98.6%,与人和鼠的C aspase-3基因核苷酸、氨基酸序列同源性分别为88.6%、83.5%和87.8%、86.7%,而且猪、人、鼠的C aspase-3上的催化和剪切位点都极为保守。

Cloning and sequence analysis of Caspase-3 gene of pig

Caspase is a kind of proteases which is related to apoptosis,and Caspase-3 is the ultimate execute in apoptosis.In order to study the biological function of Caspase-3,the Caspase-3 gene of pig was amplified by RT-PCR with the extraction of RNA from PK-15 cells.After cloning and sequencing,the results showed that the nucleotide and the deduced amino acid sequences of cloned Caspase-3 shared 98.9% and 98.6% homology with those of Caspase-3 published in GenBank.As compared with that of human and mouse,the Caspase-3 gene homology of the nucleotide and the deduced amino acid sequences were 88.6%,83.5% and 87.8%,86.7%,respectively.The active sites corresponding catalysis and cleavage on Caspase-3 among the pig,human and mouse were conservative very much.