牡丹休眠枝离体培养鳞芽萌动率的测定

[目的]了解牡丹芽体的生长发育,为其组织培养或促成栽培提供参考。[方法]选取牡丹早、中、晚花3种类型,共41个品种,将其带顶芽的枝条在去离子水中培养,从2007年12月21日到2008年1月26日,从形态学角度进行观察。[结果]3个品种的萌动率均呈上升趋势,其中早花品种的萌动率最高且在观察期间一直呈上升趋势,中花品种次之,晚花品种最低。早花品种中"凤丹白"的萌动率最高,达到100%;中花品种中"天香湛露"的萌动率最高,达到80%;晚花品种中"胡红"的萌动率最高,达到80%。[结论]在相同冷量条件下,早花品种最先萌动,芽发育良好,中花品种次之,最后是晚花品种。

Determination on the Germinating Rate of Bulbils on the Dormant Shoots of Peony in Vitro Culture

[Objective] The research aimed to in vestigate the growth and development of peony buds so as to supply references for its tissue culture and forcing cultivation.[Method] Forty-one varieties in 3 types of early,middle and late flowering peonies were selected and their shoots with top buds were cultured in deionized water.From Dec.21,2007 to Jan.26,2008,their morphology was observed.[Result] The germinating rates of the 3 varieties showed increasing trend,among them that of early-flowering varieties was highest and always showed increasing trend during observation,that of middle-flowering varieties was secondary and that of late-flowering varieties was lowest.The germinating rate of Fengdanbai among early flowering varieties was highest,reaching 100%,that of Tianxiangzhanlu among middle flowering varieties was highest and that of Huhong among late-flowering varieties was highest and both of them reached 80%.[Conclusion] Under the same cooling capacity,the early flowering varieties germinated earliest,with well developed buds,the middle flowering varieties germinated secondarily and the late flowering varieties germinated at last.