Characterisation of a novel ilarvirus causing grapevine angular mosaic disease |
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| Authors: | Sobhi M. Girgis Frederic P. Bem Chrysostomos I. Dovas Athanassios Sclavounos Apostolos D. Avgelis Mina Tsagris Nikos Katis Panayota E. Kyriakopoulou |
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| Affiliation: | (1) Laboratory of Biotechnology of Agricultural Products-Institute of Technology of Agricultural Products, National Agricultural Research Foundation, 14123 Lykovryssi, Athens, Greece;(2) Laboratory of Virology, Benaki Phytopathological Institute, 14561 Kiphissia, Athens, Greece;(3) Laboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece;(4) Department of Plant Pathology, Agricultural University of Athens, 11855 Athens, Greece;(5) Plant Virus Laboratory, National Agricultural Research Foundation, 71110 Herakleion, Crete, Greece;(6) Institute of Molecular Biology and Biotechnology, 71110 Herakleion, Greece;(7) Department of Biology, University of Crete, 71000 Herakleion, Crete, Greece;(8) Laboratory of Plant Pathology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; |
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| Abstract: | Unusual symptoms were observed on ‘Baresana’ x ‘Baresana’ Vitis vinifera hybrid vines in the Grapevine Variety Collection of the Grapevine Institute, Athens. The affected vines showed sharp angular mosaic on leaves, along the veins and in vein angles, malformations, abortive flowers or very few berries with smaller, wrinkled and non-germinating seeds, as well as gradual decline, severe stunting and death of the vine. Serological tests on diseased vines for the presence of 13 known grapevine viruses gave negative results. An infectious agent was transmitted mechanically to several herbaceous indicator plants. Koch’s Postulates were fulfilled, and the agent, proven to be a virus, was named Grapevine angular mosaic virus (GAMV). Serological tests have been developed for the virus. The most conserved polymerase region showed significant similarity of GAMV with members of subgroup 1 of the Ilarvirus genus; however ML phylogenetic analysis could not support its clustering within this subgroup. GAMV differs serologically and in particle morphology from Grapevine line pattern virus (GLPV) a putative member of the Ilarvirus genus that infects grapevine. It is proposed that GAMV is a novel member of the Ilarvirus genus. |
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