添加氯化钾和蛋黄对虹鳟精液冷冻保存的效果

为筛选出更有效的虹鳟(Oncorhynchus mykiss)精液冷冻保存液,在以10％ 甲醇和0.3 M葡萄糖作为主要成分的基础上,分别或同时添加0.9 g/L氯化钾和10％ 蛋黄形成四种冷冻保存液,用于虹鳟精液冷冻保存试验,分别形成4个处理组:Ⅰ#(不添加氯化钾和蛋黄)、Ⅱ#(添加氯化钾)、Ⅲ#(添加蛋黄)和Ⅳ#(...

Effect of potassium chloride and egg yolk on cryopreservation of rainbow trout semen

In order to screen out a more effective rainbow trout(Oncorhynchus mykiss)semen cryopreservation solution,on the basis of 10%methanol and 0.3 M glucose as the main components,0.9 g/L potassium chloride and 10%egg yolk were added respectively or simultaneously to form four cryopreservation solutions for rainbow trout semen cryopreservation test.Four treatment groups were formed respectively:I^#(no potassium chloride or egg yolk),II^#(adding potassium chloride),Ⅲ^#(adding egg yolk)andⅣ^#(adding potassium chloride and egg yolk).The sperm activation ratio,survival time and sperm viability of rainbow trout frozen sperm among the groups were compared.The results showed that the sperm viability of frozen semen in group I^#was the highest,which was 24.59%,however,no significant difference was found compared with those of the other three groups,and the activation ratio of frozen semen of this group was also the highest(24.00%±9.67%),which was significantly higher than those of groupⅢ^#andⅣ^#,but no significant difference with that of groupⅡ^#.The average survival time of frozen sperm in groupⅣ^#was the longest,which was 80.3 s.Therefore,it is speculated that the addition of potassium chloride or egg yolk decreased the activation ratio and sperm viability of frozen semen of rainbow trout;the addition of potassium chloride improved the sperm survival time to a certain extent.In conclusion,the addition of potassium chloride and egg yolk respectively or in combination may reduce the cryopreservation effect of rainbow trout semen.I^#cryopreservation solution without potassium chloride and egg yolk is recommended for the cryopreservation of rainbow trout semen.