| 小麦黄花叶病毒罗田分离物细胞质内含体蛋自基因的序列分析 |
| |
| 引用本文： | 耿波,韩成贵,李大伟,于嘉林,刘仪.小麦黄花叶病毒罗田分离物细胞质内含体蛋自基因的序列分析[J].植物病理学报,2003(2). |
| |
| 作者姓名： | 耿波 韩成贵 李大伟 于嘉林 刘仪 |
| |
| 作者单位： | 中国农业大学，植物病理学系和农业生物技术国家重点实验室，中国农业大学，植物病理学系和农业生物技术国家重点实验室，中国农业大学，植物病理学系和农业生物技术国家重点实验室，中国农业大学，植物病理学系和农业生物技术国家重点实验室，中国农业大学，植物病理学系和农业生物技术国家重点实验室 北京 100094，北京 100094，北京 100094，北京 100094，北京 100094 |
| |
| 基金项目： | “973”项目(D2000016201)，国家自然科学基金(39870028和39870489) |
| |
| 摘 要： | 利用RT-PCR,获得了小麦黄花叶病毒湖北罗田分离物细胞质内含体(CI)蛋白基因的cDNA克隆。序列分析结果表明,湖北罗田分离物CI基因由1977个核苷酸组成,编码一个由659个氨基酸组成的蛋白质。与已报道的河南潢川、四川雅安、江苏扬州及日本分离物序列比较,不同分离物之间核苷酸序列同源性在95.0％～97.5％之间,相应推导的氨基酸序列同源性在93.2％～97.1％之间。并对CI蛋白的功能进行了讨论。
|
| 关 键 词： | 小麦黄花叶病毒 CI蛋白基因 cDNA克隆 序列分析 |
Sequence analysis of the cylindrical inclusion protein gene of Luotian isolate of Wheat yellow mosaic virus |
| |
| GENG Bo,HAN Cheng-gui,LI Da-wei,YU Jia-lin,LIU Yi State Key Laboratory for Agrobiotechnology and.Sequence analysis of the cylindrical inclusion protein gene of Luotian isolate of Wheat yellow mosaic virus[J].Acta Phytopathologica Sinica,2003(2). |
| |
| Authors: | GENG Bo HAN Cheng-gui LI Da-wei YU Jia-lin LIU Yi State Key Laboratory for Agrobiotechnology and |
| |
| Institution: | GENG Bo,HAN Cheng-gui,LI Da-wei,YU Jia-lin,LIU Yi State Key Laboratory for Agrobiotechnology and Department of Plant Pathology,China Agricultural University,Beijing 100094,China |
| |
| Abstract: | A cDNA clone containing cylindrical inclusion (CI) protein gene from Luotian isolate of Wheat yellow mosaic
virus (WYMV) in Hubei Province was obtained by RT-PCR, and the nucleotide sequence of the CI gene was determined.
The CI gene of this isolate comprised 1977 nucleotides and encoded 659 amino acids containing consensus nucleotide
binding motif. When the CI gene sequence of the isolate was compared with those of previously reported WYMV isolates
from Huangchuan in Henan, Ya' an in Sichuan, Yangzhou in Jiangsu and Japan, the nucleotide and amino acid sequence
similarities ranged between 95. 0% and 97. 5 %, and between 93. 2% and 97. 1%, respectively. The function of CI protein
was also discussed in this paper. |
| |
| Keywords: | wheat yellow mosaic virus cylindrical inclusion protein gene cDNA cloning sequence analysis |
| 本文献已被 CNKI 等数据库收录！ |
