| 圈养狼繁殖期粪便中孕酮、雌二醇的水平变化 |
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| 引用本文： | 沙未来,张洪海,陈磊. 圈养狼繁殖期粪便中孕酮、雌二醇的水平变化[J]. 林业研究, 2010, 21(3): 367-372,399. DOI: 10.1007/s11676-010-0083-2 |
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| 作者姓名： | 沙未来 张洪海 陈磊 |
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| 作者单位： | [1]中科院西北高原生物研究所,青海西宁810001 [2]曲阜师范大学生命科学学院,山东曲阜273165 [3]中科院研究生院,北京100039 [4]东北林业大学,黑龙江哈尔滨150040 |
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| 基金项目： | Program for New Century Excellent Talents，Shandong Province Natural Science Foundation，Project of Science and Technology Development Plan in Shandong Province，Shandong Province Science Fund for Distinguished Young Scholars |
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| 摘 要： | 为了探索狼发情行为的生理基础,2005年12月至2006年4月,在哈尔滨北方森林动物园对参与繁殖的四只雌性狼采用非损伤性方法收集动物粪便。通过粪样激素抽提,利用化学衍生技术,对抽提样品进行衍生,采用高效液相色谱分析法（HPLC）,成功实现对狼粪样中的类固醇激素进行分离,结果表明：雌狼发情前雌二醇（E2）的含量一直处于较低水平,进入发情后含量会迅速升高至峰值且发情期间在较高的水平波动,这与多数哺乳动物发情期E2出现峰值的现象基本一致,峰值的出现即是雌狼发情的高潮期的来临。发情期E2浓度显著高于非发情期（p〈0.01）,P浓度在整个孕期也显著升高（P〈0.01）,四只雌狼均成功与配对的雄狼发生交配现象,并在当繁殖季节成功产仔。
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| 关 键 词： | 狼 粪便类固醇 雌二醇 孕酮 发情 妊娠 |
| 收稿时间： | 2010-07-02 |
Fecal progesterone and estradiol changes during the breeding season in captive female wolf |
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| Wei-lai Sha,Hong-hai Zhang,Lei Chen. Fecal progesterone and estradiol changes during the breeding season in captive female wolf[J]. Journal of Forestry Research, 2010, 21(3): 367-372,399. DOI: 10.1007/s11676-010-0083-2 |
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| Authors: | Wei-lai Sha Hong-hai Zhang Lei Chen |
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| Affiliation: | (1) Division of Environmental and Ecological Engineering, Purdue University, 500 Central Dr., West Lafayette, IN 47907, USA;(2) Department of Biology, Illinois Wesleyan University, PO. Box 2900, Bloomington, IL 61702, USA;(3) Department of Chemistry, Illinois Wesleyan University, PO. Box 2900, Bloomington, IL 61702, USA;(4) Department of Environment and Natural Resources, Government of Northwest Territories, Box 2668, Yellowknife, NT, X1A 2P9, Canada;(5) Montana Department of Fish, Wildlife and Parks, 1420 E Sixth St., Helena, MT 59620, USA;(6) Alaska Department of Fish and Game, 1300 College Rd., Fairbanks, AK 99701, USA;(7) Idaho Department of Fish &; Game, 16569 S 10th Ave, Caldwell, ID 83607, USA;(8) APHIS Wildlife Services, 9134 W. Blackeagle Dr., Boise, ID 83709, USA;(9) U.S. Fish and Wildlife Service, 585 Shepard Way, Helena, MT 59601, USA;(10) Present address: Wildlife Conservation Society, 6075 Quail Meadows Ct, Reno, NV 89519, USA |
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| Abstract: | Understanding basic reproductive physiology is crucial for the management of both captive and free-ranging wolf. In the present study,we determined hormonal changes during pregnancy and the estrous cycle in captive female wolf by measuring fecal steroids collected during the breeding season with high performance liquid chromatography (HPLC).These biochemical analyses were validated using chemical devivatization and mass spectrometry, and interpreted along with the behavioral data.All four females undergoing estrus cycles were copulated with their partners and delivered pups successfully. We found that estradiol concentrations were significantly higher during the estrus cycle than other stages (p＜0.01) and progesterone was also significantly increased throughout the pregnancy (p＜0.01). These hormonal fluctuations demonstrated pregnancy-specific changes in the fecal progesterone and estradiol concentrations. Patterns of fecal estradiol and progesterone concentrations during estrous cycles were similar to those reported for other canids. |
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| Keywords: | Canis lupus estradiol estrus fecal steroid pregnancy progesterone |
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