Inheritance and identification of DNA markers associated with yellow berry tolerance in wheat (Triticum aestivum L.)

Yellow berry (YB) is a serious seed disorder in durum wheat, bread wheatand triticale, which arises due to deficiency in nitrogen concentration in thesoil. YB seriously affects the grain protein content (GPC) thereby affectingbread making quality in bread wheat and pasta making quality in durumwheat. In order to study the inheritance and to identify DNA markersassociated with YB tolerance, a recombinant inbred line (RIL) populationof 113 individuals was developed by making a cross between RyeSelection111 (RS111), highly resistant to YB and Chinese Spring (CS), asusceptible parent. Phenotyping of this population to YB incidenceindicated that, at least one major gene/QTL and few minor genes governthe tolerance to YB. DNA marker analysis revealed linkage of twomicrosatellite markers Xgwm174 and Xgwm190 from chromosome 5Dwith YB tolerance while one ISSR marker UBC842₆₀₀ and oneRAPD marker OPR8₁₀₀₀ from chromosome 6B were found to beassociated with YB tolerance in repulsion phase. Association of YBtolerance with that of GPC was analyzed using the markers associated withYB tolerance. It was found to be reciprocal in this population in accordancewith the previous reports.