川翠3号黄瓜SSR指纹图谱的构建及种子纯度鉴定

为了建立杂交黄瓜品种川翠3号的快速纯度鉴定体系,用164对全基因组覆盖的SSR引物对川翠3号及其亲本进行了多态性筛选及纯度鉴定。其中4对引物表现出稳定的共显性,电泳图谱清晰、易分辨,特异性强,对川翠3号种子鉴定纯度为99.0%,真、假杂种编号与田间鉴定结果一致,可以用来区别其中混杂的母本、父本及其他种子,说明这4对引物组成的指纹图谱能为川翠3号杂交种的真伪鉴定、纯度检测及亲本提纯等提供准确的技术指导。

Fingerprint Construction and Purity Identification with SSR Marker for cucumber Cultivar Chuancui No.3

In order to establish fast purity identification system for hybrid cucumber cultivar Chuancui No.3 seed, we used 164 pairs SSR primers which covered whole genome for purity identification and polymorphic analysis of cucumber cultivar Chuancui No.3 and its parents. The results showed that, four primers were stably codominant, their electrophoretogram were clear, easy to distinguish, and had high specialty, its molecular marker identification purity was 99.0% by detecting some cucumber cultivar Chuancui No.3 seeds, which was the same as the field identification result,they could be used to distinguish medley female parent, male parent and other seeds. So, the fingerprint with these four pairs primers could provide more accurate technical guidance for authenticity identification, purity test and parents purification of hybrids.