Determination of baseline susceptibility of European populations of Tuta absoluta (Meyrick) to indoxacarb and chlorantraniliprole using a novel dip bioassay method |
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Authors: | Emmanouil Roditakis Christina Skarmoutsou Marianna Staurakaki María del Rosario Martínez‐Aguirre Lidia García‐Vidal Pablo Bielza Khalid Haddi Carmelo Rapisarda Jean‐Luc Rison Andrea Bassi Luis A Teixeira |
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Affiliation: | 1. National Agricultural Research Foundation (NAGREF), Plant Protection Institute of Heraklion, , Heraklion, Heraklion, Greece;2. Departamento de Producción Vegetal, Universidad Politécnica de Cartagena (UPCT), , Cartagena, Spain;3. Department of Agri‐food and Environmental Systems Management, Applied Entomology Section, University of Catania (UC), , Catania, Italy;4. DuPont de Nemours ERDC, , Nambsheim, France;5. DuPont Italia Srl, , Cernusco sul Naviglio, MI, Italy;6. DuPont Crop Protection, Stine Haskell Research Center, , Newark, DE, USA |
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Abstract: | BACKGROUND: Tuta absoluta(Meyrick) is one of the most serious pests of tomato recently introduced in the Mediterranean region. A novel bioassay method designed for the accurate determination of insecticide toxicity on T. absoluta (IRAC method No. 022) was validated by three different laboratories [Greece (NAGREF), Italy (UC) and Spain (UPCT)] on European populations. RESULTS: The insecticides indoxacarb and chlorantraniliprole were used as reference products. The IRAC leaf dip method is easy to perform, producing repeatable, homogeneous responses. LC50 values for indoxacarb ranged between 1.8 and 17.9 mg L?1 (NAGREF), 0.93 and 10.8 mg L?1 (UC) and 0.20 and 0.70 mg L?1 (UPCT), resulting in a tenfold, 12‐fold and fourfold difference between the least and most susceptible populations at each laboratory respectively. For chlorantraniliprole, LC50 values ranged between 0.10 and 0.56 mg L?1 (NAGREF), 0.23 and 1.34 mg L?1 (UC) and 0.04 and 0.24 mg L?1 (UPCT), resulting in a sixfold difference in all three cases. Overall, UPCT reported lower mean LC50 to indoxacarb, while UC reported higher LC50 to chlorantraniliprole. CONCLUSIONS: The new bioassay is reliable, providing a useful tool in the design of IRM strategies. Within each country/lab, the variability observed in the results for both indoxacarb and chlorantraniliprole can be attributed to natural variation. Future research is necessary to determine the extent to which it is possible to compare results among laboratories. Copyright © 2012 Society of Chemical Industry |
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Keywords: | Tuta absoluta indoxacarb chlorantraniliprole baseline toxicity leaf dip assay Greece Italy Spain |
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