| 桑天牛卵啮小蜂孤雌生殖及体内沃尔巴克氏体(Wolbachia)的检测 |
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| 引用本文： | 王达,黄大庄,袁芳芳,王志刚,杨威,阎海霞.桑天牛卵啮小蜂孤雌生殖及体内沃尔巴克氏体(Wolbachia)的检测[J].蚕业科学,2007,33(3):448-451. |
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| 作者姓名： | 王达 黄大庄 袁芳芳 王志刚 杨威 阎海霞 |
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| 作者单位： | 1. 河北农业大学,保定,071000
2. 承德林业调查规划设计院,承德,067000 |
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| 基金项目： | 河北省林业局科技项目(编号0534302) |
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| 摘 要： | 桑天牛卵啮小蜂(Aprostocetus prolixus)有孤雌生殖现象,其后代几乎为雄性。沃尔巴克氏体(Wolbachia)是广泛分布于节肢动物体内的一类共生菌,参与多种调控寄主的生殖活动机制。通过对外膜蛋白基因(wsp)、细菌细胞分裂蛋白基因(ftsZ)和核糖体16S rDNA基因的特异性扩增,在米蛾(Corcyra cephalonica Stainton)的DNA中分别扩增出约600、1000和900 bp的片断,验证了米蛾体内已感染了Wolbachia;而在桑天牛卵啮小蜂的DNA中未扩增出任何片断,表明Wolbachia在桑天牛卵啮小蜂体内未被感染或感染率极低。
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| 关 键 词： | 桑天牛卵啮小蜂 孤雌生殖 分子检测 沃尔巴克氏体 |
| 文章编号： | 0257-4799（2007）03-0448-04 |
| 修稿时间： | 2007-03-26 |
Parthenogenesis and Molecular Detection of the Wolbachia in the Aprostocetus prolixus |
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| WANG Da,HUANG Da-Zhuang,YUAN Fang-Fang,WANG Zhi-Gang,YANG Wei,YAN Hai-Xia.Parthenogenesis and Molecular Detection of the Wolbachia in the Aprostocetus prolixus[J].Acta Sericologica Sinica,2007,33(3):448-451. |
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| Authors: | WANG Da HUANG Da-Zhuang YUAN Fang-Fang WANG Zhi-Gang YANG Wei YAN Hai-Xia |
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| Institution: | 1. Agricultural University of Hebei, Baoding Hebei 071000, China; 2. Chengde Forestry Survey and Planning Design Institute, Chengde Hebei 067000, China |
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| Abstract: | Aprostocetus prolixus has a phenomenon of parthenogenesis and its offspring are almost male.Wolbachia is a common and widespread group of bacteria found in arthropods.These bacteria evolve various mechanisms for manipulating reproduction of their hosts.Based on the amplification of the wsp,ftsZ and 16S rDNA gene fragments of 600 bp,1 000 bp and 900 bp in length,respectively from the DNA of a laboratory population of Corcyra cephalonica Stainton,it is proved that the Corcyra cephalonica Stainton has been infected with the Wolbachia;But no any fragment from the DNA of the Aprostocetus prolixus was amplified,suggesting that the Aprostocetus prolixus was not infected by Wolbachia or the infection rate was extremely low. |
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| Keywords: | Aprostocetus prolixus Parthenogenesis Molecular detection Wolbachia |
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