黄河鲇鱼的同工酶研究

[目的]为我国黄河鲇鱼的研究提供依据。[方法]以30-60日龄的黄河鲇鱼和鲤鱼为试验材料，分别取鱼体肌肉、脑、肝、消化道、眼、肾、性腺、心各0.4g，用双蒸水冲洗干净，冰浴研磨，离心后取上清液用于电泳分析，根据酶的结构组成和同工酶在各组织中所表现的酶谱，确定每种同工酶的编码座位数和座位的等位基因数。[结果]黄河鲇的同工酶在不同组织中表现出不同程度的差异，即同工酶在同一个体不同组织中表达的类型和程度不同；不同组织的同工酶酶谱染色深浅不同，酶的活性大小存在差异。[结论]黄河鲇种群具有广泛的遗传变异和遗传多样性。

Study on Isozyme of Silurus lanzhouensis in Yellow River

[Objective] The aim was to provide the basis for studying Silurus lanzhouensis in yellow river.[Method] With S.lanzhouensis and Cyprinus carpio at 30-60 day-old as the tested materials,their muscle,brain,liver,digestive tract,eye,kidney,gonad and heart were selected for 0.4 g and were washed with double distilled water.After grinding in ice bath and centrifugation the supernatant was taken for electrophoresis.According to the structural composition of enzyme and zymogram of isozyme in tissues,the number of ...