| 狐狸脑炎的免疫效果监测(英文) |
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| 引用本文： | 史秋梅,高光平,高桂生,张艳英,张东林,卢会鹏,王晓珊,韩明媚.狐狸脑炎的免疫效果监测(英文)[J].农业科学与技术,2014,15(12):2175-2177. |
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| 作者姓名： | 史秋梅 高光平 高桂生 张艳英 张东林 卢会鹏 王晓珊 韩明媚 |
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| 作者单位： | 河北科技师范学院,河北省预防兽医学重点实验室,河北昌黎066600 |
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| 基金项目： | Key Technology Research and Development Program of Hebei Province，Key Technology Research and Development Program of Shijiazhuang Municipal Science and Technology Bureau，Fund from Department of Education of Hebei Province，Fund from Qinhuangdao Academy of Agricultural Sciences (2014-04).河北省科技支撑计划，河北省石家庄市科技局科技支撑计划，河北省教育厅项目，秦皇岛市农业科学院项目 |
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| 摘 要： | 目的]检测昌黎地区狐狸脑炎的免疫效果。方法]从昌黎县泥井一养殖小区随机选择4个养殖户,85份取狐狸全血,制备血清,利用ELISA方法检测狐狸脑炎抗体,根据ELISA结果,计算样品实际浓度并判定其(CAV-1)抗体的存在与否。结果]通过对85份血清样品的检测,4户养殖户狐狸脑炎免疫情况分别为:养殖户1的15份血清中,14例阳性,1例阴性,免疫合格率达93%;养殖户2的14份血清中,12例阳性,2例阴性,免疫合格率达到86%;养殖户3的30份血清中,29例阳性,1例阴性,免疫合格率达到97%;养殖户4的26份血清中,24例阳性,2例阴性,免疫合格率达到92%。该次检测免疫总合格率为93%。结论]昌黎地区狐狸脑炎免疫效果良好,个别狐狸需要加强免疫。
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| 关 键 词： | 狐狸脑炎 抗体 ELISA |
Survey on Vaccination of Foxes Against Encephalitis |
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| Qiumei SHI,Guangping GAO,Guisheng GAO,Yanying ZHANG,Donglin ZHANG,Huipeng LU,Xiaoshan WANG,Mingmei HAN.Survey on Vaccination of Foxes Against Encephalitis[J].Agricultural Science ＆ Technology,2014,15(12):2175-2177. |
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| Authors: | Qiumei SHI Guangping GAO Guisheng GAO Yanying ZHANG Donglin ZHANG Huipeng LU Xiaoshan WANG Mingmei HAN |
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| Institution: | ( Hebei Provincial Key Laboratory of Veterinary Preventive Medicine, College of Animal Science, Hebei Normal University of Sci- ence and Technology, Changli 066600, China) |
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| Abstract: | Objective] This study was conducted to survey the vaccination of foxes against encephalitis in Changli. Method] A total of 85 fox blood samples were col- lected from four farmers in Changli County. The serum antibody titer against fox encephalitis was detected by enzyme-linked immunosorbent assay （ELISA）. The OD values were measured to determine the presence of CAV-1 antibody in samples. Result] 93% of the 15 serum samples from Farmer 1, 86% of the 14 serum sam- ples from Farmer 2, 97% of the 30 serum samples from Farmer 3 and 92% of 26 serum samples from farmer 4 were CAV-1 antibody-positive. In summary, among the 85 serum samples from the four farmers, six were CAV-1 antibody-negative, and 79 were CAV-1 antibody-positive, namely 93% of the foxes were successfully immunized. Conclusion] The results revealed that vaccination against fox encephali- tis in Changli was run well, but the immunization in several foxes should be strengthened. |
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| Keywords: | Fox encephalitis Antibody ELISA |
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