Characteristics of a resistance-breaking isolate of potato virus Y causing potato tuber necrotic ringspot disease |
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| Authors: | J F J M van den Heuvel R A A van der Vlugt M Verbeek P T de Haan H Huttinga |
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| Institution: | (1) Present address: DLO Research Institute for Plant Protection (IPO-DLO), P.O. Box 9060, 6700 GW Wageningen, The Netherlands;(2) Department of Virology, Wageningen Agricultural University, P.O. Box 8045, 6700 EM Wageningen, The Netherlands;(3) S & G Seeds Research, P.O. Box 26, 1600 AA Enkhuizen, The Netherlands |
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| Abstract: | An Austrian isolate of potato virus YNTN, the causal agent of potato tuber necrotic ringspot disease (PTNRD), was serologically compared with seven Dutch PVYN isolates. Using polyclonal and monoclonal antibodies, it was found indistinguishable from PVYN. Determination of the nucleotide sequence of the coat protein cistron and comparison of the deduced amino acid sequence with coat protein sequences of other potyviruses revealed a high level of homology with PVYN coat protein sequences. This confirmed the close taxonomic relationship of PVYNTN with the PVYN subgroup of potato virus Y. PVYNTN is able to overcome all resistance genes known so far in commercial potato cultivars. Remarkably, transgenic PVY-protected tobacco plants are also resistant to PVYNTN infection upon mechanical and aphid-mediated inoculation. These experiments indicate that genetically engineered resistance offers great potential in protection of potato to new aggressive strains of PVYN. |
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| Keywords: | Potyviridae potato virus Y detection monoclonal antibodies ELISA virus transmission aphids transgenic resistance |
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