Reduction in vigour of leafless potato cuttings in vitro |
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Authors: | Janet E A Seabrook L Katheryn Douglass |
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Institution: | (1) Agriculture and Agri-Food Canada Research Centre, P.O. Box 20280, E3B 4Z7 Fredericton, New Brunswick, Canada |
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Abstract: | Summary Removing the leaves from single node potato cuttings in vitro allows propagules to be placed in culture vessels at increased
densities with the intent of saving resources and space. Under light intensities of 22 wattsm−2, the removal of the subtending leaf resulted in fewer nodes, shorter plantlets, smaller leaf area, and lower fresh and dry
weights of 4-week-old plantlets of cvs Atlantic, Kennebec, Russet Burbank and Shepody. Fewer nodes and reduced plantlet height
may result in inefficient multiplication protocols because fewer propagules are available and shorter internodes make dissection
more difficult. The coefficient of variation for the various growth parameters was greater when propagules lacked leaves,
indicating that the variability of the plant material for propagation was increased. A reduction of vigour and growth was
still evident when plantlets of cv. Shepody were grown for 6–8 weeks. |
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Keywords: | Solanum tuberosum L multiplication micropropagation growth leaf removal tissue culture variation |
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