| 粘质沙雷氏菌发酵产红色素培养基及其结构研究 |
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| 引用本文： | 薛建,刘青,杨启银.粘质沙雷氏菌发酵产红色素培养基及其结构研究[J].安徽农业科学,2009,37(12):5375-5378. |
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| 作者姓名： | 薛建 刘青 杨启银 |
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| 作者单位： | 南京师范大学生命科学学院,江苏南京,210046;南京师范大学生命科学学院,江苏南京,210046;南京师范大学生命科学学院,江苏南京,210046 |
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| 摘 要： | 目的]对红色素生产菌粘质沙雷氏菌的培养基进行优化。方法]以粘质沙雷氏菌RZ-21为供试菌种，研究培养基中碳源、氮源种类及用量对菌体生物量和红色素产量的影响。结果]以黄豆粉为碳源时，菌体生物量和红色素产量分别为15．994和1．551g/L，分别为基础培养基（牛肉膏为碳源）的1．72和1．91倍；以酪素和蚕蛹粉为氮源时，菌体生物量分别为18．755和19．873g／L，分别为基础培养基（以蛋白胨为氯源）的2．04和2．16倍，红色素产量分别为2．025和1．744g／L，分别为基础培养基的2．51和2．17倍；全波长扫描和质谱分析结果显示，该红色素为灵菌红素。结论]粘质沙雷氏菌发酵生产红色素的最佳培养基为：黄豆籼．5％，酪素1％，蚕蛹粉1％，K2HPO40．2％，NaCl0．5％，此条件下，菌体生物量和色素产量分别为26．587和2．642g／L。
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| 关 键 词： | 培养基 生物量 灵菌红素 发酵 |
Study on Medium for Serratia marcescens Fermenting and Producing Red Pigment and the Structure of Red Pigment |
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| XUE Jian et al.Study on Medium for Serratia marcescens Fermenting and Producing Red Pigment and the Structure of Red Pigment[J].Journal of Anhui Agricultural Sciences,2009,37(12):5375-5378. |
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| Authors: | XUE Jian |
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| Institution: | XUE Jian et al(Life Sciences College of Nanjing Normal University,Nanjing,Jiangsu 210046) |
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| Abstract: | Objective] The aim was to optimize the medium for red pigment producing strain Serratia marcescens RZ-21.Method] With S.marcescens RZ-21 as the tested strain,the effects of different kinds of carbon resource and nitrogen resource in medium on the biomass yield and red pigment yield were studied.Result] When carbon resource was soybean flour,the biomass yield and red pigment yield were 15.994 and 1.551 g/L resp.,being 1.72 and 1.91 times of those in basis medium(beef extract as carbon resource).When nitro... |
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| Keywords: | Medium Biomass Prodigiosin Fermentation |
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