| 湖南省山羊脑多头蚴的线粒体nad1和nad4基因的序列测定及种系发育分析 |
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| 引用本文： | 何德肆,徐平源,彭运潮. 湖南省山羊脑多头蚴的线粒体nad1和nad4基因的序列测定及种系发育分析[J]. 畜牧兽医学报, 2011, 42(12) |
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| 作者姓名： | 何德肆 徐平源 彭运潮 |
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| 作者单位： | 湖南生物机电职业技术学院,长沙,410128 |
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| 基金项目： | 湖南省教育厅项目(09C1209); 湖南养殖科研项目(201009) |
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| 摘 要： | 本研究旨在阐明脑多头蚴湖南分离株线粒体烟酰胺腺嘌呤二核苷酸( NADH)脱氢酶亚单位1基因(nad1)部分序列(pnad1)和烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位4基因(nad4)部分序列(pnad4)的遗传变异情况,并用pnad1和pnad4序列重构脑多头蚴与其它带科绦虫的种群遗传关系.利用聚合酶链反应(PCR)扩增脑多头蚴的pnad1和pnad4,应用ClustalX 1.81程序对序列进行比对,再用Phylip3.67程序MP法和Mage4.0程序NJ法绘制种系发育树,并用Puzzle5.2程序构建最大似然树,同时利用DNAstar5.0中的Megalign程序进行同源性分析.结果显示所获得的pnad1和pnad4序列长度分别均为666和887 bp,湖南分离株与已知多头带绦虫位于同一分枝.由于脑多头蚴pnad1和pnad4序列种内相对保守,种间差异较大,故均可作为种间遗传变异研究的标记,从而为脑多头蚴的分子流行病学和其相关疾病的诊断奠定基础.
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| 关 键 词： | 多头带绦虫 线粒体DNA nad1基因 nad4基因 种系发育关系 |
Sequence and Phylogenetic Analysis of Mitochondrial nad1 and nad4 Genes for Coenurus cerebralis in Goats in Hunan Province |
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| HE De-si , XU Ping-yuan , PENG Yun-chao. Sequence and Phylogenetic Analysis of Mitochondrial nad1 and nad4 Genes for Coenurus cerebralis in Goats in Hunan Province[J]. Chinese Journal of Animal and Veterinary Sciences, 2011, 42(12) |
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| Authors: | HE De-si XU Ping-yuan PENG Yun-chao |
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| Affiliation: | HE De-si,XU Ping-yuan,PENG Yun-chao (Hunan Biological and Electromechanical Polytechnic College,Changsha 410128,China) |
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| Abstract: | The objectives of the present study were to examine sequence variation in the mitochondrial NADH dehydrogenase subunit 1(nad1) gene and NADH dehydrogenase subunit 4(nad4) gene among Coenurus cerebralis isolates in Hunan Province,and to reconstruct their phylogenetic relationship using nad1 and nad4 sequences.The partial nad1(pnad1) and nad4(pnad4) were amplified from each C.cerebralis,and pnad1 and pnad4 sequences were aligned using the ClustalX 1.81.MP,and NJ trees of pnad1 and pnad4 were constructed using... |
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| Keywords: | C.cerebralis mitochondrial DNA nad1 gene nad4 gene phylogenetic relationship |
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