杉木和秃杉群体的叶绿体微卫星分析

对５个杉木和７个秃杉天然群体进行胚的叶绿体微卫星分析，每个群体分析了１２￣３６粒种子，试用了两种ＤＮＡ提取方法，这两种方法获得的ＤＮＡ数量和质量相仿，可以采用较为经济的方法。研究了各种ＤＮＡ扩增条件并获得了合适的条件组合。应用Ａｒｌｅｑｕｉｎ和ＰＨＹＬＩＰ软件分析了群体分子变异模式及群体遗传关系，结果表明叶绿体微卫星方法能够有效地分析出杉木和秃杉的群体以及树种内的变异情况，对于研究针对树种的进化历

STUDY ON GENETIC DIVERSITY OF CUNNINGHAMIA LANCEOLATA AND TAIWANIA FLOUSIANA BY USING CHLOROPLAST MICROSATELLITES

DNA was isolated from germinating embryos of 5 and 7 natural populations of \%Cunninghamia lanceolata\% and \%Taiwania flousiana\%, respectively. Two different techniques were used for the DNA isolation. Similar results in terms of quantity and quality of DNA were obtained and the economical one was selected as main method. The best DNA amplification condition was obtained after experiments. AMOVA was studied by using the software of Arlequin and PHYLIP. Results showed that the cpSSR was sensitive in revealing the genetic variation between 2 tree species and among the populations inside species, it is of special importance in studing the evolution history of coniferous tree species, gene resources conservation strategy and tree breeding strategy.