4种柑橘衰退病毒源单蚜传毒分离株CP基因的分子特征

 对4种柑橘衰退病毒源及其31个单蚜传毒分离株的CP基因做了限制性片段长度多态性(RFLP)和单链构象多态性(SSCP)分析,并对其中8个单蚜传毒分离株的CP基因进行了序列分析。实验明确了所研究柑橘衰退病毒(Citrus tristeza virus,CTV)的CPG/Hinf I RFLP组群和CPG/SSCP模式,二者能较好地对应并有效验证了单蚜传毒对CTV毒源的分离纯化作用;CP基因序列分析结果表明,相同CPG/Hinf I RFLP组群的单蚜传毒分离株间具有高度的序列相似性,而不同CPG/Hinf I RFLP组群单蚜传毒分离株间则存在较大差异;通过与已知生物学特性CTV分离株比较,初步建立了上述CP基因分子特征与病毒生物学特性之间的联系。

Molecular characteristics of CP genes of 4 isolates of Citrus tristeza virus and their sub-isolates after single aphid transmission

Restriction fragment length polymorphism(RFLP) and single strain conformation polymorphism(SSCP) assays on coat protein(CP) genes of 4 CTV-isolates and their 31 sub-isolates after single aphid transmission were conducted,and CP genes of the 8 sub-isolates were sequenced.CPG/Hinf I RFLP groups and SSCP patterns of all the CTV were discovered,and both demonstrated that single aphid transmission was an effect way to separate CTV isolates.The results of sequencing showed that CP genes of sub-isolates with the characteristic of the same RFLP group had high homomorphism,while these fallen into different groups had lower homomorphism.The relationship among these molecular characteristics of CP genes and biological cha-racters of virus were preliminarily suggested by comparison with identified CTV isolates based upon typical symptoms.