云南、福建、湖南烟区烟草花叶病主要病毒种类检测及黄瓜花叶病毒亚组鉴定

 采用抗原直接包被和双抗体夹心酶联免疫吸附测定法(ELISA)对采自云南、福建、湖南烟区烟草花叶病样品进行了病毒种类检测,利用三抗体夹心ELISA对黄瓜花叶病毒(Cucumber mosaic virus,CMV)的亚组类型进行了鉴定。在云南采集的520个花叶病样品中,烟草花叶病毒(Tobacco mosaic virus,TMV)、CMV和马铃薯Y病毒(Potato virus Y,PVY)总检出率分别为71.74%、55.01%和6.35%;在福建采集的150个花叶病样品中,TMV、CMV和PVY的总检出率分别为94%、24.66%和8.00%;在湖南采集的74个花叶病样品中,TMV、CMV和PVY的总检出率分别为58.11%、51.35%和2.70%。部分样品为2种以上病毒复合侵染。云南、福建和湖南采集的64个CMV阳性样品中,属亚组Ⅰ的样品为57个,占89.1%;属亚组Ⅱ的样品为10个,占15.6%;其中3个样品为亚组Ⅰ和亚组Ⅱ的复合侵染。

Virus detection of tobacco mosaic disease and subgrouping of Cucumber mosaic virus (CMV) in Yunnan, Fujian and Hunan Provinces

Tobacco leaf samples with mosaic symptoms collected from Yunnan, Fujian and Hunan Provinces were tested for virus types by using antigen direct coated or double antibody sandwich enzyme-linked immunosorbent assay(ELISA), and triple antibody sandwich(TAS) ELISA was then used for subgrouping of Cucumber mosaic virus(CMV) samples. Among 520 tobacco samples collected in Yunnan Province, 71.74%, 55.01% and 6.35% samples were infected by Tobacco mosaic virus(TMV), CMV and Potato virus Y(PVY), respectively. Among 150 tobacco samples collected in Fujian Province, 94%, 24.66% and 8.00% samples were infected by TMV, CMV and PVY, respectively. Among 74 tobacco samples collected in Hunan Province, 58.11%, 51.35% and 2.70% samples were infected by TMV, CMV and PVY, respectively. Some samples were infected with more than two viruses. Among 64 CMV samples, 89.1% samples were infected with subgroupⅠisolates and 15.6% were infected with subgroupⅡ isolates, three samples were infected with both subgroupⅠand subgroupⅡ isolates.