CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo |
| |
Authors: | Konev Alexander Y Tribus Martin Park Sung Yeon Podhraski Valerie Lim Chin Yan Emelyanov Alexander V Vershilova Elena Pirrotta Vincenzo Kadonaga James T Lusser Alexandra Fyodorov Dmitry V |
| |
Institution: | Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA. |
| |
Abstract: | The organization of chromatin affects all aspects of nuclear DNA metabolism in eukaryotes. H3.3 is an evolutionarily conserved histone variant and a key substrate for replication-independent chromatin assembly. Elimination of chromatin remodeling factor CHD1 in Drosophila embryos abolishes incorporation of H3.3 into the male pronucleus, renders the paternal genome unable to participate in zygotic mitoses, and leads to the development of haploid embryos. Furthermore, CHD1, but not ISWI, interacts with HIRA in cytoplasmic extracts. Our findings establish CHD1 as a major factor in replacement histone metabolism in the nucleus and reveal a critical role for CHD1 in the earliest developmental instances of genome-scale, replication-independent nucleosome assembly. Furthermore, our results point to the general requirement of adenosine triphosphate (ATP)-utilizing motor proteins for histone deposition in vivo. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|