Distribution of the introduced cyprinid herpesvirus 3 in a wild population of common carp, Cyprinus carpio L. |
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Authors: | K Uchii K Matsui T Iida Z Kawabata |
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Affiliation: | Research Institute for Humanity and Nature, Kitaku, Kyoto, Japan; Department of Civil and Environmental Engineering, Kinki University, Higashiosaka, Osaka, Japan; National Research Institute of Aquaculture, Fisheries Research Agency, Minami-Ise, Mie, Japan |
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Abstract: | Cyprinid herpesvirus 3 (CyHV-3), which causes a lethal disease in common carp, Cyprinus carpio L., and koi, C. carpio koi , first occurred in Lake Biwa, Japan in 2004. To elucidate distribution of CyHV-3 in a wild common carp population, we conducted a PCR survey of CyHV-3 among such fish in Lake Biwa in 2006. Only 6% (1/18) of the common carp smaller than 300 mm were positive with PCR, whereas 31% (18/58) of fish larger than 300 mm were positive. To evaluate their past exposure to CyHV-3 infection based on the presence of antibodies, we also measured the levels of serum anti-CyHV-3 antibodies in the carp, using an enzyme-linked immunosorbent assay. None (0/26) of the fish smaller than 300 mm was positive for the antibodies, whereas 54% (33/61) of fish larger than 300 mm were positive. Of the antibody-positive individuals, 44% (14/32) were also positive by PCR strongly suggesting that wild common carp that survived infection become CyHV-3 carriers. Five individuals were positive by PCR but negative for antibodies indicating that their infection with CyHV-3 had occurred recently. These results suggest that transmission of CyHV-3 from carriers to naïve common carp is still occurring in Lake Biwa. |
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Keywords: | antibody carrier common carp cyprinid herpesvirus 3 koi herpesvirus |
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