氟唑活化酯诱导大白菜抗根肿病效果与机理初步研究

为明确新型植物诱导抗病剂氟唑活化酯对大白菜根肿病的诱导抗病效果及抗病机理，研究了氟唑活化酯的诱导浓度、在白菜根部的运输过程以及氟唑活化酯诱导白菜后相关防御基因的表达情况。结果显示以氟唑活化酯25 mg ? L-1对大白菜进行叶面喷雾诱导时根肿病的病情指数最低。通过台盼蓝（Trypan blue）染色观察到氟唑活化酯诱导大白菜叶片后，根部对根肿菌也产生了一定的抗性。利用Real-time PCR技术检测大白菜防御相关基因的表达，发现氟唑活化酯诱导大白菜2 h后JA途径中的COI1和LOX2基因以及SA途径中的PR-1基因都有显著的过量表达，其中JA信号途径相关基因表达变化更明显。结果说明氟唑活化酯可以诱导大白菜产生系统获得抗性，两种信号传导途径都参与了抗病过程，但以JA途径为主，SA途径为辅。

Systemic Resistance Induced by Fluoro-substituted Benzothiadiazole Derivatives Against Plasmodiophora brassicae in Chinese Cabbage

In this study，a novel inducer similar to Benzothiadiazole（BTH）structurally，namely Fluoro-substituted benzothiadiazole derivatives（FBT）was investigated to determine the mechanisms of resistance against Plasmodiophora brassicae in Chinese cabbage. The leaves were pretreated by foliar spraying with FBT at different concentrations，disease severity was suppressed most at 25 mg ? L-1. We stained the roots with trypan blue，found that the root colonization by Plasmodiophora brassicae were significantly controlled after the leaves were treated with FBT. Analysis of defense-related genes by Real-time PCR showed that FBT excites the expression of SA-responsive PR-1 gene and JA-responsive COI1，LOX2 genes obviously，indicating that the JA-signaling pathway is essential for FBT-induced resistance in Chinese cabbage，including partial SA-signaling.