Cloning and characterization of repetitive sequences and development of SCAR markers specific for the P genome of <Emphasis Type="Italic">Agropyron cristatum</Emphasis> |
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| Authors: | M Wu J P Zhang J C Wang X M Yang A N Gao X K Zhang W H Liu L H Li |
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| Institution: | (1) The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 100081 Beijing, People’s Republic of China;(2) College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, 712100 Yangling, Shanxi Province, People’s Republic of China |
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| Abstract: | In previous studies, we successfully transferred the P genome of Agropyron cristatum into wheat using wide hybridization methods. In the current investigation, repetitive sequences were cloned and DNA markers
specific for the P genome of A. cristatum were developed. Three P genome-specific markers, designated OPX07-1036, OPX11-817 and OPC05-1539, were identified and isolated
using random amplified polymorphic DNAs. The three markers were successfully amplified in all tested materials that contained
Agropyron chromatin, such as Agropyron itself and wheat-Agropyron addition lines. These RAPD markers were converted into SCAR markers to be used in detection of P genome chromatin in wheat.
In situ probing with fluorescent-labeled marker DNA has shown that they are distributed in all arms of Agropyron hence they will be useful in a variety of studies on introgressions of the P-genome chromatin into wheat. |
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