Study on the Expression of FBP1 Gene in AA Broilers under Cold Stimulating Environment

In order to study of FBP1 gene expression under cold stimulating environment,75 AA broilers as research object were selected and randomly assigned to 5 groups,one of group was control group that raising in normal temperature,the remaining four groups were cold stimulation groups that raising temperature was 3 ℃ lower than the control group.The time of cold stimulation in four groups were 1,3,5 and 24 h,respectively.Cold stimulation started at 8 days of age,ended at 21 days of age.Broilers were slaughtered at 22 days of age and investigated FBP1 gene expression in each group.The results showed that the expression of FBP1 gene existed tissue specificity under cold stimulating environment.The relative expression level of FBP1 gene in liver was significantly higher than the other tissues in 1 h group (P< 0.05);The relative expression level of FBP1 gene in lung were significantly higher than the other tissues except of liver in 3 h group except for liver (P< 0.05);The relative expression level of FBP1 gene in liver were significantly higher than the other tissues in 5 h group (P< 0.05);The relative expression level of FBP1 gene in thymus were significantly higher than the other tissues in 24 h group (P< 0.05).FBP1 gene expression inside the heart was on the decline in the same organization different treatment groups as the rime of cold stimulating was increasing except for 3 h group.FBP1 gene expression of liver in 5 h group was significantly higher than other FBP1 gene expression in each treatment group.FBP1 gene expression of spleen was significant decreased in 1 h group,the other group extend a downward trend with the extension of cold stimulation time.FBP1 gene expression in lung was downward trend after rising first.FBP1 gene expression in kidney was significantly decreased in 24 h group,and the other groups had no obvious change.FBP1 gene expression of thymus in 24 h group was significantly higher than the other groups (P< 0.05),and there was no significant difference between control group and other four terament groups (P >0.05).