p38 MAPK/ATF-2 pathway is involved in C-relative protein-induced endothelial cell activation |
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Authors: | LIU Shao-jun LI Yuan-mei LIU Wei-hua XIONG Long-gen LIU Shi-ming |
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Affiliation: | 1. Department of Cardiology, Guangzhou Institute of Cardiovascular Disease, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China;2. College of Life Science and Bio-pharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China |
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Abstract: | AIM: To investigate the role of p38 MAPK/ATF-2 pathway in C-relative protein (CRP)-induced endothelial cell activation. METHODS: Human coronary artery endothelial cells (HCAEC) were cultured and were used between passages 3 and 7. CRP served as a stimulus for endothelial cell activation. Western blotting was performed to determine the expression and phosphorylation of eNOS, p38 and ATF2. ELISA was carried out to detect the levels of ICAM-1, VCAM-1 and MCP-1 released from HCAEC. Pharmacological p38 inhibitors SB203580 and SB202190 were used to determine the effect of p38/ATF-2 pathway. RESULTS: CRP reduced the p-eNOS level in a concentration-dependent manner and induced the release of ICAM-1, VCAM-1 and MCP-1. The p38/ATF-2 pathway was activated by CRP treatment. SB203580 and SB202190 partially rescued p-eNOS level and suppressed the secretion of ICAM-1, VCAM-1 and MCP-1. CONCLUSION: p38MAPK/ATF-2 pathway participates in CRP-induced endothelial activation. |
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Keywords: | p38 MAPK/ATF-2 pathway C-relative protein Endothelial activation Atherosclerosis |
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