Limb ischemic preconditioning protects human umbilical vein endothelial cells from oxidative stress induced by H2O2 |
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Authors: | CHEN Min ZHANG Ming-sheng ZHANG Xuan-ping LIANG Yue-qin |
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Affiliation: | 1. Department of Pharmacology,Shanxi Medical University, Taiyuan 030001, China;2. Medical Functional Experimental Center, Shanxi Medical University, Taiyuan 030001, China |
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Abstract: | AIM: To investigate the effects of the sera from the rats after limb ischemic preconditioning (LIPC) on human umbilical vein endothelial cells (HUVECs) injured by hydrogen peroxide (H2O2). METHODS: The HUVECs were divided into 5 groups: the cells in control group were cultured without any intervention; the cells in model group (M) were damaged by 1 mmol/L H2O2 for 2 h; the cells in early preconditioning serum (EPS) group, delayed preconditioning serum (DPS) group or sham limb ischemic preconditioning serum (SPS) group were treated with the corresponding serum at 5% for 12 h, respectively, and then treaed with H2O2 for 2 h. The viability of the HUVECs was analyzed by flow cytometry. The lactate dehydrogenase (LDH) in the culture media was detected. The cell adhesion molecules in the HUVECs were detected by real-time PCR. The mRNA and protein expression of heme oxygenase-1 (HO-1) was also determined. RESULTS: The viability of HUVECs incubated with 1 mmol/L H2O2 for 2 h significantly decreased compared with the control cells, which was accompanied with the augmentations of LDH in the medium and the cell adhesion molecules in cells, such as vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). Preincubation with EPS and DPS derived from the rats subjected LIPC attenuated these injuries. Furthermore, pretreatment with EPS and DPS increased the expression of HO-1 at mRNA and protein levels. CONCLUSION: LIPC protects the HUVECs from H2O2-induced injury. |
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Keywords: | Limb ischemic preconditioning Human umbilical vein endothelial cells Oxidative stress |
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