Cloning and Sequence Analysis of ORF1 Gene of Porcine Torque Teno sus Virus Type 1a |
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Authors: | CHEN Peng-qiang HU Chong-wei LIN Qun-qun CHEN Qiu-yong XIU Jin-sheng |
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Institution: | College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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Abstract: | In order to know the ORF1 gene of the Fujian isolates of porcine Torque teno sus virus (PTTSuV) type 1a,the study amplified the ORF1 gene of PTTSuV type 1a from the DNA isolated in the feces with diarrhea in Fujian province.The target PCR fragments were cloned and sequenced,and spliced with SeqMan software.The results showed that the cloned ORF1 gene of PTTSuV type 1a was 1 947 bp in length,coding an open reading frame (ORF) with 648 amino acids,its theoretical isoelectric point was 10.06.The nucleotide sequence and the amino acid sequence deduced from the gene were analyzed by the bioinformatics software.Compared with the PTTSuV type 1a ORF1 gene from GenBank,the sequenced gene shared the highest homology with TTV1 Bj2-1 strain (GenBank accession number:HM633243) at 99.7%.The phylogenetic tree was constructed with Mega 6.06,the PTTSuV type 1a could be divided into 2 different phylogenetic clusters (clusterⅠ and cluster Ⅱ),the cluster Ⅰ also had two distinguish branch (Ⅰa and Ⅰb).The results of this study could enrich the genomic characterization of PTTSuV type 1a in Fujian. |
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Keywords: | porcine Torque teno sus virus type 1a ORF1 gene clone sequence analysis |
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