赤芍水提物与抗菌药联合对耐药大肠杆菌抑菌作用的研究

为了探究赤芍水提物与抗菌药联合对耐药大肠杆菌的体外抑菌作用,采用微量肉汤稀释法与微量棋盘法来测定各抗菌药与赤芍水提物的最低抑菌浓度(MIC)及二者联合作用后的分级抑菌浓度指数(FICI)。结果显示赤芍水提物与头孢曲松钠、阿莫西林、环丙沙星、诺氟沙星、左旋氧氟沙星、磷霉素、痢菌净、克林沙星、磺胺间甲氧嘧啶、加替沙星、阿米卡星、头孢他啶、林可霉素、头孢噻呋钠、氟苯尼考、阿奇霉素、头孢噻肟、利福平联合作用后的FICI分别为0.27、2.50、1.02、1.01、3.00、0.28、1.02、1.02、1.02、2.02、1.02、0.75、0.56、0.75、0.50、3.00、0.75、0.38。结果表明赤芍水提物与头孢曲松钠、磷霉素、氟苯尼考、利福平联合作用时有协同作用;与林可霉素、头孢噻呋钠、头孢噻肟、头孢他啶联合作用时有相加作用;与阿莫西林、左旋氧氟沙星、加替沙星、阿奇霉素联合作用时有颉颃作用;与环丙沙星、诺氟沙星、痢菌净、克林沙星、磺胺间甲氧嘧啶、阿米卡星联合作用时表现为无关作用。

Study on the Anti-bacterial Effects of Aqueous Extract of Radix Paeoniae Rubra Combined with Antibiotics against Drug-resistant E.coli

The assay was aimed to explore the combination of aqueous extract of Radix Paeoniae Rubra and antibiotics against drug-resistant E.coli, we used the broth micro-dilution method and micro-dilution checkerboard technique to determine the minimum inhibiotory concentration (MIC) and fractional inhibitory concentration index (FICI).The results showed that the FICI of aqueous extract of Radix Paeoniae Rubra combined with ceftriaxone sodium, amoxicillin, ciprofloxacin, norfloxacin, levofloxacin, fosfomycin, mequindox, clinafloxacin, sulfamonomethoxine, gatifloxacin, amikacin, ceftazidime, lincomycin, ceftiofur sodium, florfenicol, azithromycin, cefotaxime and rifampicin were 0.27, 2.50, 1.02, 1.01, 3.00, 0.28, 1.02, 1.02, 1.02, 2.02, 1.02, 0.75, 0.56, 0.75, 0.50, 3.00, 0.75 and 0.38, respectively.The results indicated that the aqueous extract of Radix Raeoniae Rubra combined with ceftriaxone sodium, fosfomycin, florfenicol and rifampin had synergy effects;Combined with lincomycin, ceftiofur sodium, cefotaxime and ceftazidime had additive effect;Combined with amoxicillin, levofloxacin, gatifloxacin and azithromycin had antagonism effect;Combined with ciprofloxacin, norfloxacin, mequindox, clinafloxacin, sulfamonomethoxine and amikacin showed independent action.