Mechanism of Herceptin enhancing apoptosis and improving sensitivity to chemotherapy in Ishikawa cells |
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Authors: | SHEN Yuan LU Lin WANG Xiao-yu |
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Institution: | 1. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China;
2. Obstetrics & Gynecology Department, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China |
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Abstract: | AIM: To investigate the role of Herceptin in the apoptosis and drug sensitivity of endometrial cancer Ishikawa cells.METHODS: The IC50 values of Herceptin, adriamycin(ADR), cisplatin(DDP) and paclitaxel(PTX) for Ishikawa cells were detected by MTT method. Ishikawa cells were treated with single drug and combined chemotherapy for 24 h, the cell cycle and the apoptosis ratio were determined by flow cytometry.RESULTS: The IC50 values of Herceptin, ADR, DDP and PTX were 57.12 mg/L, 0.572 μmol/L, 67.4 μmol/L and 719.5 nmol/L, respectively. Herceptin significantly enhanced the cytotoxicity of the chemotherapeutic drugs, and increased apoptosis ratio statistically.CONCLUSION: Herceptin enhances the apoptosis-inducing ability of the chemotherapeutic drugs and improves the chemotherapeutic sensitivity in Ishikawa cells. |
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Keywords: | Herceptin Endometrial cancer Chemotherapeutic sensitivity Apoptosis |
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