Adiponectin attenuates H2O2-induced SH-SY5Y cell injury and tau hyperphosphorylation via activating PP2A |
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Authors: | SONG Zhe XUE Chao ZHANG Xiao-man ZHANG Ye-min HE Xiao-hua YIN Jun |
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Affiliation: | School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China |
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Abstract: | AIM: To study the effects of adiponectin on H2O2-induced cell injury and tau hyperphosphorylation in human neuroblastoma SH-SY5Y cells. METHODS: Cell viability was determined by MTT assay. H2O2-induced cell injury and morphological changes in the SH-SY5Y cells with or without adiponectin treatment were observed. The level of tau phosphorylation as well as the activities of protein phosphatase 2A(PP2A) and of glycogen synthase kinase-3β(GSK-3β) were examined by Western blotting. RESULTS: Adiponectin significantly attenuated H2O2-induced cell injury(P<0.01). Adiponectin upregulated the activity of PP2A and decreased phosphorylation levels of tau under the stimulation with H2O2 (P<0.01). Okadaic acid, a specific inhibitor of PP2A, blocked the protective effects of adiponectin(P<0.01). Adiponectin increased the phosphorylation level of GSK-3β at Ser9 site under H2O2 stimulation(P<0.01). CONCLUSION: Adiponectin protects SH-SY5Y cells against H2O2-induced cell injury and decreases tau hyperphosphorylation by activating PP2A and inactivating GSK-3β. |
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Keywords: | Adiponectin Hydrogen peroxide Protein phosphatase 2A Glycogen synthase kinase-3β Tau proteins |
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