Roles of maspin in biological behaviors of A549 cells

AIM: To investigate the roles of maspin in the biological behaviors of non-small-cell lung cancer A549 cells.METHODS: Full-length coding region of maspin was amplified by PCR from human normal breast tissue and cloned into MSCV vector. Virus supernatants was produced by Phoenix A packaging system, and target cell line was infected with the virus supernatants. The transfected cells were screened with puromycin. The cell line with maspin over-expression was identified by real-time PCR and Western blot. Cell growth, migration and invasion were investigated by xCelligence system.RESULTS: Maspin over-expression vector was successfully constructed. The cell line with maspin over-expression was established. No difference of the growth between A549-control cells and A549-maspin cells was observed. The migration and invasion were quite different between A549-control cells and A549-maspin cells. The expression level of integrin β1 in A549-maspin cells was decreased compared with A549-control cells.CONCLUSION: Maspin has no effect on the growth of A549 cells. Maspin suppresses the migration and invasion of A549 cells, in which integrin β1 is involved.