Effect of Different Concentrations of Neuropeptide P on Expression of BMP2 and BMP4 Genes in Skin Stem Cells of Mongolia Cashmere Goat

This study was aimed to explore the expression of BMP2 and BMP4 genes in skin stem cells of Mongolia Cashmere goat with the influence of different concentrations of neuropeptide P (SP).We set five different SP concentrations (0, 1×10^-6, 1×10^-7, 1×10^-8 and 1×10^-9 mol/L) to stimulate the skin stem cells, then identified the expression quantities of BMP2 and BMP4 at days of 0, 7, 14 and 21 by Real-time quantitative PCR with the purpose of determining the optimum SP concentration for the differentiation of skin stem cells.The results showed that SP with the concentrations of 1×10^-6 and 1 ×10^-8 mol/L promoted the expression of BMP2 and BMP4;The expression of BMP2 at 7 and 14 d in SP 1×10^-8 mol/L group was significantly higher than that of the SP 1×10^-6 mol/L group (P<0.05);The expression of BMP4 in SP 1×10^-6 mol/L group had no significant difference with the SP 1×10^-8 mol/L group (P>0.05);At 21 d, the expression of BMP2 and BMP4 significantly decreased in SP 1×10^-8 and the SP 1×10^-6 mol/L groups, and the expression of BMP2 in the SP 1×10^-8 mol/L group was significantly higher than that of the 1×10^-6 mol/L group (P<0.05), while the expression of BMP4 in the SP 1×10^-6 mol/L group had no significant difference with that of SP 1×10^-8 mol/L group (P>0.05).The results indicated that the concentrations of 1×10^-6 and 1×10^-8 mol/L not only might affect the expression of BMP2 and BMP4 of skin stem cells, but also provided a theoretical basis for the directional differentiation of skin stem cells.