Effect of Different Concentrations of Neuropeptide P on Expression of BMP2 and BMP4 Genes in Skin Stem Cells of Mongolia Cashmere Goat |
| |
Authors: | WANG Ya-juan GAO Feng LIU Ying-chun ZHOU Huan-min |
| |
Institution: | 1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. College of Animal Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China;
3. Key Laboratory of Inner Mongolia Autonomous Region Biological Manufacturing, Hohhot 010018, China |
| |
Abstract: | This study was aimed to explore the expression of BMP2 and BMP4 genes in skin stem cells of Mongolia Cashmere goat with the influence of different concentrations of neuropeptide P (SP).We set five different SP concentrations (0, 1×10-6, 1×10-7, 1×10-8 and 1×10-9 mol/L) to stimulate the skin stem cells, then identified the expression quantities of BMP2 and BMP4 at days of 0, 7, 14 and 21 by Real-time quantitative PCR with the purpose of determining the optimum SP concentration for the differentiation of skin stem cells.The results showed that SP with the concentrations of 1×10-6 and 1 ×10-8 mol/L promoted the expression of BMP2 and BMP4;The expression of BMP2 at 7 and 14 d in SP 1×10-8 mol/L group was significantly higher than that of the SP 1×10-6 mol/L group (P<0.05);The expression of BMP4 in SP 1×10-6 mol/L group had no significant difference with the SP 1×10-8 mol/L group (P>0.05);At 21 d, the expression of BMP2 and BMP4 significantly decreased in SP 1×10-8 and the SP 1×10-6 mol/L groups, and the expression of BMP2 in the SP 1×10-8 mol/L group was significantly higher than that of the 1×10-6 mol/L group (P<0.05), while the expression of BMP4 in the SP 1×10-6 mol/L group had no significant difference with that of SP 1×10-8 mol/L group (P>0.05).The results indicated that the concentrations of 1×10-6 and 1×10-8 mol/L not only might affect the expression of BMP2 and BMP4 of skin stem cells, but also provided a theoretical basis for the directional differentiation of skin stem cells. |
| |
Keywords: | skin stem cells neuropeptide P BMP expression |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|