Protective effect of Auricularia auricular-judae polysaccharide on pulmonary tissues of rats with LPS-induced acute lung injury |
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Authors: | GAO Yang LIU Bin Hanizaier REHEMAN LI Zhuan-yun SUN Zhan |
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Institution: | 1. Clinical College, Xinjiang Medical University, Urumqi 830054, China;
2. Preclinical Medical College, Xinjiang Medical University, Urumqi 830054, China |
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Abstract: | AIM: To investigate the effects of Auricularia auricular-judae polysaccharide(AAP) on pulmonary tissues of rats with LPS-induced acute lung injury(ALI) and its mechanisms.METHODS: Adult Sprague-Dawley rats were randomly divided into control group, LPS group,low-dose AAP group, middle-dose AAP group, high-dose APP group, and dexamethasone group. The rats were injected with LPS(8 mg/kg, ip) to induce ALI. The rats in the AAP groups were treated with AAP for 7 d before the induction of ALI. The protein concentration in the bronchoalveolar lavage fluid(BALF) was measured. The lung edema degree was measured by detecting the wet/dry weight ratio. The myeloper-oxidase(MPO), total antioxidant capacity(T-AOC), total superoxide dismutase(T-SOD), nitric oxide synthase(NOS) and malondialdehyde(MDA) levels were determined. The pathological changes of the lung tissues were evaluated by HE staining.RESULTS: Treatment with AAP significantly improved LPS-induced lung pathological changes, attenuated the protein concentration in the BALF and wet/dry weight ratio, inhibited the activities of MPO and NOS, reduced MDA level and increased the activities of T-AOC and T-SOD.CONCLUSION: AAP protects against LPS-induced acute lung injury in rats. |
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Keywords: | Auricularia auricular-judae polysaccharides Lipopolysaccharides Acute lung injury Antioxidation |
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