| 体外精子发生培养方法研究进展 |
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| 引用本文： | 纪猛,李向臣,李璐,关伟军,马月辉. 体外精子发生培养方法研究进展[J]. 中国畜牧兽医, 2015, 42(11): 3050-3055. DOI: 10.16431/j.cnki.1671-7236.2015.11.033 |
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| 作者姓名： | 纪猛 李向臣 李璐 关伟军 马月辉 |
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| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;2. 河北农业大学动物科技学院, 保定 071000 |
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| 基金项目： | 中国农业科学院科技创新工程项目(cxgc-ias-01);国家自然科学基金(31472099) |
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| 摘 要： | 近1个世纪以来,科研人员致力于研究在体外培养的条件下产生具有功能的精子。直到近几年,研究人员才成功利用体外组织培养的方法获得了具有受精能力的精子。体外研究精子发生的方法主要有组织培养法和体外培养分离细胞的方法,结合两种方法将分离的生殖细胞移植到睾丸组织中进行组织培养。作者回顾了体外精子发生的培养方法,为临床应用和阐明精子发生机制的基本问题提出相关见解。
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| 关 键 词： | 体外精子发生 器官培养 生殖细胞 干细胞 |
| 收稿时间： | 2015-04-02 |
Advance of Cultural Methods for in vitro Spermatogenesis |
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| JI Meng,LI Xiang-chen,LI Lu,GUAN Wei-jun,MA Yue-hui. Advance of Cultural Methods for in vitro Spermatogenesis[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(11): 3050-3055. DOI: 10.16431/j.cnki.1671-7236.2015.11.033 |
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| Authors: | JI Meng LI Xiang-chen LI Lu GUAN Wei-jun MA Yue-hui |
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| Affiliation: | 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China |
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| Abstract: | The generation of functional sperm in vitro has been a goal for almost a century.Fortunately,researchers reported the successful in vitro production of functional sperm,thereby potentially opening up a new era of reproductive biology.In vitro cultural methods for spermatogenesis include tissue cultural method and cells cultural method.Take together transplanting isolated cells into testicular tissue for tissue culture.Here,we summarize the history of research directed toward reproducing steps of spermatogenesis in vitro,suggest ways that can be applied toward clinical applications and address fundamental questions about the underlying mechanism of spermatogenesis. |
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| Keywords: | in vitro spermatogenesis organ culture germ cells stem cells |
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