Study on the SNP of UCP1 Gene Exon 2 in Rabbit

In order to deeply understand the meat quality and provide the basic information and scientific proof for better heredity selection of rabbit.This experiment option New Zealand rabbit and Californian rabbit were used to construct own DNA pools, respectively for complete sequences amplification of exon 2 and part of intron 1 region of UCP1 gene, two-way sequencing the amplification of the product, BLAST and DNAStar analysis and determine the SNP of rabbit UCP1 gene.The results showed that six SNPs were found in UCP1 gene of the New Zealand rabbit:T135C, C232T, T332C, C360T, T550C and A560G, two synonymous mutation (T135C and C232T) and the other SNPs located in intron region respectively.Using RNA online prediction software forecast UCP1 gene RNA secondary structure prediction software minimum free energy changed from -917.51 kJ/mol to -866.10 kJ/mol for the polymorphisms led to the changes of RNA secondary structure.