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Polymorphisms of MEF2A Gene and its Relationship with Slaughter Traits in Sansui Duck
Authors:YI Heng-jie  LI Hui  YANG Sheng-lin  PENG Bang-xing  ZHAO Zhong-hai
Institution:1. College of Animal Science, Guizhou University, Guiyang 550025, China;2. Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou Province, Guiyang 550025, China;3. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China;4. Zhengan County Livestock Industry Development Office, Guizhou Province, Zunyi 563400, China
Abstract:To understand the relationship between single nucleotide polymorphism sites (SNPs) of myocyte enhancer factor 2A (MEF2A) gene and slaughter traits in Sansui duck, a total of 60 individuals of Sansui ducks were selected to investigate in this study, direct sequencing of PCR and PCR-SSCP methods were used on single nucleotide polymorphisms of MEF2A gene, and genetic effects of its on slaughter traits were analyzed.The results showed that two SNPs which include g.47915G>A and g.47918G>A of exon 11 were found in MEF2A gene, and the G/A mutation in the g.47915G>A SNP resulted in the change of codon from GAA to AAA, and the coding amino acid from Glu to Lys, and the G/A mutation in the g.47918G>A SNP resulted in the change of codon from GAT to AAT, and the coding amino acid from Asp to Asn.The result of association of SNPs with slaughter traits showed various results were as follows:g.47915G>A and g.47918G>A had affected the eviscerated percentage.This result revealed that the polymorphism of MEF2A gene had basilic influence on slaughter traits.
Keywords:Sansui duck  myocyte enhancer factor 2A (MEF2A)  slaughter traits  
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