猪繁殖与呼吸综合征疫苗室温保存工艺的研究

为了提高猪繁殖与呼吸综合征（PRRS）弱毒活疫苗的耐热性能，用蔗糖、胰蛋白胨、EDTA、乳糖等成分配伍成不同组方，对PRRS病毒进行梯度真空泡沫干燥。37℃ 10 d耐老化试验结果表明，组方T28C-1、T28C-4、T28C-5和T28C-7的病毒损失均＜0.5 Lg；疫苗长期保存期试验结果表明，T28C-4、T28C-5在37℃可保存4个月，25℃及自然条件下可保存5个月以上；将37℃、25℃及4℃下保存3个月的T28C-4干燥疫苗免疫20日龄仔猪，免后2周ELISA抗体水平合格且与商品苗趋势相当。试验结果表明PRRSV泡沫干燥疫苗有很好的耐热效果。

Studies on Room Temperature Preservation Technology of Porcine Reproductive and Respiratory Syndrome Vaccine

To improve the heat resistance of PRRS attenuated live vaccine, several formulas composed of sucrose, tryptone, EDTA, lactose and other protectants were dried by vacuum foam along with porcine reproductive and respiratory syndrome virus. The results of aging resistance test at 37℃ for 10 d indicated that the virus titer losts were no more than 0.5 Lg in 4 formulations of T28C-1, T28C-4, T28C-5, and T28C-7. The results of vaccine long term preservation test indicated that formula T28C-4 and T28C-5 could be kept at 37℃ for 4 months, while at 25℃ and under natural conditions they could be kept for over 5 months. Besides，after preserved at 37℃、25℃ and 4℃ for 3 months, the dried vaccine T28C-4 was inoculated on piglet at 20 days of age, and 2 weeks later, the ELISA titer were all qualified and had the similar level to the commercial vaccines of lyophilization dosage.