Cloning and Sequence Analysis of VP gene of Muscovy Duck Parvovirus Zhejiang Isolate

In order to enrich the biological characteristics of VP gene from Muscovy duck parvovirus Zhejiang isolate (MDPV-ZJ),the target VP gene fragments were amplified by PCR method with specific primers,then the obtained PCR products were cloned and sequenced.The bioinformatics analysis of VP gene of MDPV-ZJ was conducted.The results revealed that MDPV-ZJ VP gene was 2 199 bp in length,coding an open reading frame (ORF) with 732 amino acids.The molecular weight,theoretical isoelectric point,instability index and grand average of hydropathicity of MDPV-ZJ VP gene were 81.32 ku,6.59,37.49 and －0.667,respectively,and with no signal peptide.The genetic evdution ary tree based on the VP gene showed that the MDPV isoaltes contains two groups:The typical MDPV and recombinant MDPV; Also,the typical MDPV could be divided into three branches:Taiwanese branch,Maniland China branch and Euro branch,which existed obvious regional genetic evolution relationship.In this assay MDPV-ZJ was belonged to MDPV Maniland China branch.