Effect of miR-142-3p on Lactation in Diary Goat Mammary Epithelial Cells |
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Authors: | WANG Chu-yue WANG Chun-mei SHAO Li ZHANG Li LIN Ye CUI Ying-jun GAO Xue-jun LI Qing-zhang |
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Institution: | Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China |
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Abstract: | To determine the regulative role of miR-142-3p in lactation of dairy goat mammary epithelia cells (DGMECs),we selected the DGMECs in lactating dairy goat for material,used liposomal transfection techniques to silence miR-142-3p expression,and used Real-time quantitative PCR,Western blotting and Assay Kits to explore the changes of lactation after miR-142-3p inhibition.The results showed that when miR-142-3p was inhibited,the cell proliferation ability of DGMECs was increased,the secretion amount of β-casein and triglyceride were up-regulated.So we concluded that miR-142-3p could influence the lactation function of DGMECs,such as cell proliferation,β-casein and triglyceride secretion,by inhibiting the function of its target genes. |
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Keywords: | diary goat mammary gland miR-142-3p cell proliferation lactation |
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