氢化物发生-原子荧光光谱法测定牛肉中的硒含量

本试验采用了氢化物发生-原子荧光光谱法测定牛肉中的硒,通过消解液、预还原剂、介质酸的选择等优化了试验条件并测定其检出限、精密度、加标回收率等。结果显示本试验选用2:1的混合酸作为消解液,6 mol/L HCl作为预还原剂,体积比为10%的HCl作为介质酸。硒在0~8 μg/L的范围内呈良好的线性关系,相关系数为0.9999,检出限为0.01 ng/mL。在20、30和40 μg/L 3个添加水平下牛肉的回收率为94%~104%,相对标准偏差(RSD)为3.80%。该方法具有简便、有效等特点,对肉制品中硒的测定具有一定的参考意义。

Detection of Selenium Content in Beef by Hydride Atomic Fluorescence Spectrometry

In this assay, we detected the selenium content in beef by hydride atomic fluorescence spectrometry.The conditions of the test were optimized by the selections of clearing up solution, pre-reducing agent, medium acid and so on, the limits of detection, precision and the standard addition recovery were determined.The results showed that 2:1 mixed acid as the clearing up solution, 6 mol/L hydrochloric acid as pre-reducing agent and the volume ratio of 10% hydrochloric acid as acid medium were chosen, the calibration curve was linear in the range from 0 to 8.0 μg/L with correlation coefficient was 0.9999.The limit of detection (LOD) was 0.01 ng/mL.At three adding levels of 20, 30 and 40 μg/L for selenium, the recovery of beef was 94% to 104% with RSD of 3.80%.This method had the characteristics of simple and effective, it had important referential significance for the detection of selenium in meat.