不同病例动物源大肠杆菌的分离鉴定及16SrDNA同源性分析

为研究不同病例发病动物的发病原因,并对不同病例病料分离出的细菌进行16S rDNA同源性分析,本试验对10种不同病例发病动物病料进行细菌分离培养并对分离获得的细菌进行微生物学鉴定,设计1对16S rDNA基因引物,对分离出的10株细菌进行PCR扩增、测序及16S rDNA同源性分析。结果显示,分离获得的10株细菌经微生物学鉴定均为大肠杆菌,10株大肠杆菌中哺乳类动物病例犬乳房炎、犬子宫蓄脓、犬肺炎、犬皮肤化脓疮、奶牛乳房炎、犊牛腹泻6株大肠杆菌之间16S rDNA同源性为100.0%,家禽类动物病例肉鸽腹泻、肉鸡腹泻、野鸡腹泻和白孔雀腹泻4株大肠杆菌之间16S rDNA同源性也为100.0%,10株不同病例动物来源大肠杆菌之间16S rDNA同源性为97.5%~100.0%。本试验探明了10种不同病例发病动物的发病原因均为大肠杆菌感染引起,且10株大肠杆菌16S rDNA之间具有高度同源性。

Isolation,Identification and 16S rDNA Homology Analysis of Escherichia coli from Animals in Different Cases

In order to study the causes of different cases,and analyze 16S rDNA homology of bacteria isolated from different cases,this study took the material of 10 different cases to isolate and culture bacteria,identify the isolates by microbiology.A pair of primers was designed to amplify the bacteria 16S rDNA gene.PCR products were sequenced and 16S rDNA homology analysis was conducted.The results showed that 10 strains bacterial were Escherichia coli through microbiology identification,6 strains of Escherichia coli from mammals cases,16S rDNA homologies among canine skin fester sores,canine pyometra,canine pneumonia,canine mastitis,dairy cow mastitis and calf diarrhea were 100.0% in 10 strains of Escherichia coli,4 strains of Escherichia coli from poultry animal cases,16S rDNA homologies among pigeon diarrhea,broiler diarrhea,pheasant diarrhea and white peacock diarrhea were also 100.0% in 10 strains of Escherichia coli;16S rDNA homologies in Escherichia coli of 10 different cases animal sources were 97.5% to 100.0%.This test proved that 10 different animals cases were caused by Escherichia coli infection,and had a high 16S rDNA homology among 10 strains of Escherichia coli.