乳酸菌发酵中药制剂防制鸡球虫病条件的优化及应用

本试验旨在摸索乳酸菌发酵抗鸡球虫中药制剂的最佳制备条件,研究其对鸡球虫病的防制效果。首先对乳酸菌发酵中药制剂的最佳菌株、接菌量、时间、温度、pH条件进行摸索,制得乳酸菌发酵中药制剂,然后选取150只AA+肉鸡进行效果试验。试验随机分为对照组、感染组、乳酸菌发酵组、西药组和纯中药组,21日龄时计算各试验组鸡生长性能及免疫细胞因子IgG、IL-12、IFN-γ、IL-10的含量。结果显示,3.0%戊糖片球菌PP培养于浓度1.0 g/mL的中药培养基,在pH 6.0、37℃的温度下培养12 h,戊糖片球菌PP的活性最高,生长良好;且乳酸菌发酵组鸡的平均日增重显著高于感染组(P<0.05),高于纯中药组及西药组,但差异不显著(P>0.05),与对照组增重效果相当(P>0.05);其料重比(F/G)显著低于感染组(P<0.05),低于纯中药组、西药组,但差异不显著(P>0.05);其IL-12、IFN-γ的表达均显著高于感染组(P<0.05),高于纯中药组和西药组,但差异不显著(P>0.05);其IL-10、IgG显著高于感染组(P<0.05),稍高于纯中药组及西药组(P>0.05)。结果表明经优化的乳酸菌发酵中药制剂可提高感染球虫鸡的生长性能,且能增强机体免疫水平。

Optimization and Application of Lactic Acid Bacteria Fermented Traditional Chinese Medicine Compound Preparations against Chicken Coccidiosis

The assay was aimed to optimize the best preparation conditions of lactic acid bacteria fermented traditional Chinese medicine preparation against chicken coccidiosis,and study the prevention and contol effect.First,explored the best lactic acid bacteria,inoculational amount,time,temperature and pH of lactic acid bacteria fermented traditional Chinese medicine preparation,and then carried out the effect experiment of 150 AA+ broilers.The broilers were randomly divided into control group,infection group,lactic acid bacteria fermented group,western medicine group and traditional Chinese medicine group,after 21 d,calculated the growth performance and cytokine levels of IgG,IL-12,IFN-γ,IL-10.The results showed that lactic acid bacteria grew well under pH 6.0,37℃ cultivated 12 h,3% P.pentosaceus PP cultivated in the concentration of 1.0 g/mL traditional Chinese medicine medium;Moreover the average daily gain of lactic acid bacteria fermented group was significantly higher than infection group (P<0.05),not sigificantly higher than traditional Chinese medicine group and western medicine group (P>0.05),equivalent to control group (P>0.05);F/G of lactic acid bacteria fermented group was significantly lower than infection group (P<0.05),not sigificantly lower than traditional Chinese medicine group and western medicine group (P>0.05);The levels of IL-12 and IFN-γ of lactic acid bacteria fermented group were significantly higher than infection group (P<0.05),not sigificantly higher than traditional Chinese medicine group and western medicine group (P>0.05);The levels of IL-10 and IgG of lactic acid bacteria fermented group were significantly higher than infection group (P<0.05),slightly higher than traditional Chinese medicine group and western medicine group (P>0.05).The results indicated that the optimized lactic acid bacteria fermented traditional Chinese medicine preparation against chicken coccidiosis could improve the growth performance and enhance the immune level of chicken.