| 药用植物仙茅的组织培养及快繁体系的建立 |
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| 引用本文： | 王任翔,李红,谢强,吴果团. 药用植物仙茅的组织培养及快繁体系的建立[J]. 安徽农业科学, 2009, 37(21): 9881-9882 |
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| 作者姓名： | 王任翔 李红 谢强 吴果团 |
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| 作者单位： | 广西师范大学生命科学学院,广西桂林,541004;广西师范大学生命科学学院,广西桂林,541004;广西师范大学生命科学学院,广西桂林,541004;广西师范大学生命科学学院,广西桂林,541004 |
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| 基金项目： | 广西师范大学引进人才和博士启动基金，广西教育厅立项项目 |
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| 摘 要： | [目的]针对仙茅市场需求量不断增长，野生资源受到严重破坏的情况，系统地研究了药用植物仙茅的组织培养及快繁体系的建立。[方法]以仙茅根茎上的幼叶为外植体，在MS培养基上添加不同的激素配比进行组织培养。[结果]外植体在MS＋6-BA0．50mg／L＋NAA0．05ms／L或MS＋6-BA1．00ms／L＋NAA0．05mg／L培养基中培养20d诱导产生愈伤组织；将诱导产生的愈伤组织转入不合激素的MS培养基中培养30d诱导产生芽，同时在芽的基部产生根；幼苗的移栽成活率高达95％以上。[结论]采用组织培养方式可进行仙茅的快速繁殖，为确保药用植物资源仙茅的保护和可持续利用提供有效途径，
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| 关 键 词： | 仙茅 愈伤组织 组织培养 快速繁殖 |
Tissue Culture and Establishment of Rapid Propagation System of Medicinal Plant Curculigo orchiodes Gaertn |
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| WANG Ren-xiang et al. Tissue Culture and Establishment of Rapid Propagation System of Medicinal Plant Curculigo orchiodes Gaertn[J]. Journal of Anhui Agricultural Sciences, 2009, 37(21): 9881-9882 |
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| Authors: | WANG Ren-xiang et al |
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| Affiliation: | WANG Ren-xiang et al (College of Life Sciences,Guangxi Normal University,Guilin,Guangxi 541004) |
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| Abstract: | [Objective] In order to preserve the natural resources of Curculigo orchiodes Gaertn which has been destroyed seriously,the method of tissue culture has been systematically studied.[Method] The explants of immature leaves were cultured on MS culture media by adding different portions of hormones at various cultural conditions.[Result] The suitable phytohormone combination to induce callus were MS+6-BA 0.50 mg/L+NAA 0.05 mg/L or MS+6-BA 1.00 mg/L+NAA 0.05 mg/L;For the adventitious buds and roots,they were MS... |
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| Keywords: | Curculigo orchiodes Gaertn Callus Tissue culture Rapid propagation |
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