| 影响大肠埃希菌感受态细胞因素的探讨 |
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| 引用本文： | 温建新,李志辉,宋明慧,周顺,任慧英,刘文华,邹玲,李迎军,王金宝.影响大肠埃希菌感受态细胞因素的探讨[J].动物医学进展,2007,28(5):9-12. |
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| 作者姓名： | 温建新 李志辉 宋明慧 周顺 任慧英 刘文华 邹玲 李迎军 王金宝 |
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| 作者单位： | 山东农业大学动物科技学院,山东泰安,271018;莱阳农学院动物科技学院,山东青岛,266109;山东省诸城市畜牧局,山东潍坊,262200;莱阳农学院动物科技学院,山东青岛,266109;山东省农业科学院,山东济南,250000 |
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| 摘 要： | 研究不同型号的菌株、不同转化溶液、感受态细胞的存放时间、转化时间及转化后的复苏时间等对质粒转化效率的影响.结果表明,转化溶液浓度和感受态细胞的存放时间对转化效率有明显的影响,而转化时间、冰浴时间和转化后的振荡培养复苏时间对转化效率的影响不明显.
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| 关 键 词： | 大肠埃希菌 感受态细胞 影响因素 |
| 文章编号： | 1007-5038（2007）05-0009-04 |
| 收稿时间： | 2007-03-06 |
| 修稿时间： | 2007年3月6日 |
Study on Impacting Factors of Escherichia coli Competent Cell Preparation |
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| WEN Jian-xin,LI Zhi-hui,SONG Ming-hui,ZHOU Shun,REN Hui-ying,LIU Wen-hua,ZOU Ling,LI Ying-jun,WANG Jin-bao.Study on Impacting Factors of Escherichia coli Competent Cell Preparation[J].Progress In Veterinary Medicine,2007,28(5):9-12. |
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| Authors: | WEN Jian-xin LI Zhi-hui SONG Ming-hui ZHOU Shun REN Hui-ying LIU Wen-hua ZOU Ling LI Ying-jun WANG Jin-bao |
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| Abstract: | A number of factors including bacterial strains, transformation solutions, freezing duration and storage duration were evaluated for their effects on transformation ability of competent Escherichia coli cells. The different effects of the transformative condition which included bacterial strains, CaClz concentration, of the competent cell status, of the various transformative time and of the incubative time on the transformative plasmid were investigated. The host cell was Escherichia coli DH5a and JM109. The resuits showed that effects of different transformation solutions and the competent cell status on the transformative frequency were obvious, but the effects of the transformative time, freezing duration and incubative time after transformation were not significant. |
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| Keywords: | Escherichia coli competent cells impacting factors |
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