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| 蛋白质电泳在豌豆品种鉴定中的应用 | |
| 引用本文： | 孙雁,朱有勇,朱永平,杨琼珍. 蛋白质电泳在豌豆品种鉴定中的应用[J]. 种子, 2004, 23(2): 24-26,30 |
| 作者姓名： | 孙雁 朱有勇 朱永平 杨琼珍 |
| 作者单位： | 1. 云南农业大学,云南省植物病理重点实验室,昆明,650201 2. 云南农业大学农学与生物技术学院,昆明,650201 3. 云南省个旧市种子公司,个旧,661400 |
| 摘 要： | 以25个豌豆品种为材料,分别用重蒸水2.5%NaCl、0.2%NaOH和75%乙醇提取其种子蛋白,利用连续型乳酸-聚丙烯酰胺凝胶电泳进行分离,结果表明:豌豆贮藏蛋白以水溶、盐溶和碱溶为主,醇溶蛋白甚微.三种主要蛋白在品种间存在不同程度的谱带差异,均可用于豌豆的品种鉴定,但以水溶和碱溶蛋白的谱带差异最大,是豌豆品种鉴定的首选蛋白.连续型乳酸-PAGE方法简便快速、分离效果好,是一种经济有效的方法. |
| 关 键 词： | 豌豆 种子 贮藏蛋白 乳酸-PAGE 品种鉴定 |
Application of Proteins Gel Electrophoresis in The Identification of Pea Cultivars |
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| Sun yan Zhu Youyong Zhu Yongping Yang Qiongzhen. Application of Proteins Gel Electrophoresis in The Identification of Pea Cultivars[J]. Seed, 2004, 23(2): 24-26,30 | |
| Authors: | Sun yan Zhu Youyong Zhu Yongping Yang Qiongzhen |
| Affiliation: | Sun yan1 Zhu Youyong1 Zhu Yongping2 Yang Qiongzhen3 |
| Abstract: | Seed proteins of 25 pea cultivars were respectively extracted using distilled H 2O, 2.5%NaCl, 0.2%NaOH and 75%alcohol. Based on coherent lactate polyacrylamide gel electrophoresis, the results showed that most of storage proteins were water-soluble, salt-soluble and alkali-soluble proteins. There is a little alcohol-soluble protein. All the three main proteins with polymorphism PAGE-banding patterns can be used in cultivars identification of pea, The salt-soluble and alkali-soluble proteins are more suitable to be used as biochemical marks of pea cultivars than salt-soluble. It also demonstrated that coherent lactate PAGE is a effective method in identification of pea cultivars, which is simple, rapid, inexpensive and has a good effect on protein isolation. |
| Keywords: | Pea seeds Storage protein Lactate polyacrylamide gel electrophoresis Cultivars identification |
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