| 生防菌株HJX1的鉴定和抑菌谱的测定 |
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| 引用本文： | 赵更峰,邱逸敏,张云霞,熊国如. 生防菌株HJX1的鉴定和抑菌谱的测定[J]. 商界．城乡致富, 2014, 0(4): 19-22 |
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| 作者姓名： | 赵更峰 邱逸敏 张云霞 熊国如 |
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| 作者单位： | [1]海南医学院理学院,海南海口571199 [2]中国热带农业科学院热带生物技术研究所,海南海口571101 |
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| 基金项目： | 海南医学院2013年科研培育基金资助（HY2013-22）. |
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| 摘 要： | 依据传统形态学、16S rRNA基因序列及脂肪酸图谱鉴定，将一株对香蕉镰刀枯萎病菌有很好抑制效果的菌株HJX1鉴定为枯草芽孢杆菌。采用细菌的通用引物P0和P6扩增16S rRNA基因片段，得到1521bp的DNA片段，其序列与枯草芽孢杆菌的序列的同源性高达99%；细胞组分脂肪酸的含量同枯草芽孢杆菌最近，其相似度为0.724。与病原真菌对峙培养的结果表明，该菌株能对供试的10个植物病原真菌均有很好的抑制作用。
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| 关 键 词： | 枯草芽孢杆菌HJX1 鉴定 香蕉枯萎病 生物防治 |
Identification and Fungistatic Effect of Biocontrol Strain HJX1 |
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| Affiliation: | ZHAO Gengfeng et al (College of Science, Hainan Medical College, HaiKou 571199, China) |
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| Abstract: | A biocontrol strain HJX1, which had good inhibitory effect against Fusarium oxysporum f.sp. cubense, was identified as Bacillus subtilis based on morphology, 16S rRNA gene sequence and fatty acid profiles. The sequence of 16S rRNA gene had 99% identity to B. subtilis according to the DNA fragment of 1521bp amplified with a pair of universal primers, P0 and P6, of bacteria. Confront culture with pathogenic fungi showed that this strain had good inhibitory effect against 10 pathogenic fungi. |
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| Keywords: | Bacillus subtilis HJX1 Identification Fusarium oxysporum f.sp.cubense Biological control |
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