Covert LdMNPV detected in Lymantria dispar larvae during a survey of potential hosts for DekiNPV production |
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| Authors: | M M Yang W J Zhai B C Li W Zhang Zh Q Zhang M L Li |
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| Institution: | 1. Department of Biology, Institute of Bioengieering, Shaanxi Xueqian Normal University, Shaanxi, P.R. China;2. The Key Laboratory of Forest Protection, State Forestry Administration of China, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, P.R. China;3. Forestry Entomology Laboratory of Academic Forestry, College of Forestry, Northwest Agriculture and Forestry University, Shaanxi, P.R. China;4. Forestry Entomology Laboratory of Academic Forestry, College of Forestry, Northwest Agriculture and Forestry University, Shaanxi, P.R. China |
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| Abstract: | Dendrolimus kikuchii nucleopolyhedrovirus (DekiNPV) is a biological control agent used to control the populations of D. kikuchii, which is a pest of variety of conifers, including Pinus kesiya, Pinus fenzeliana, Cedrus deodara, and Keteleeria evelyniana. However, D. kikuchii cannot be artificially reared which restricts the production and utilization of DekiNPV. In this study, the infectivity and replication of DekiNPV was demonstrated in Spodoptera frugiperda cell lines (sf9), Clostera anachoreta cell line (CAF-clan II), and Lymantria dispar (L.) to identify suitable alternative hosts for the production of DekiNPV. We also investigated the ability of DekiNPV to trigger the conversion of covert infections to overt infections. Results showed the absence of DekiNPV in the two cell lines and in L. dispar. However, polymerase chain reaction results indicated that L. dispar larvae harbored covert infection that could not be triggered by DekiNPV. |
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| Keywords: | nucleopolyhedrovirus covert infection Dendrolimus kikuchii Spodoptera frugiperda cell line (sf9) Clostera anachoreta cell line (CAF-clan II) biological control |
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