Synthetic mammalian prions |
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Authors: | Legname Giuseppe Baskakov Ilia V Nguyen Hoang-Oanh B Riesner Detlev Cohen Fred E DeArmond Stephen J Prusiner Stanley B |
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Institution: | Institute for Neurodegenerative Diseases, University of California, San Francisco, CA 94143, USA. |
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Abstract: | Recombinant mouse prion protein (recMoPrP) produced in Escherichia coli was polymerized into amyloid fibrils that represent a subset of beta sheet-rich structures. Fibrils consisting of recMoPrP(89-230) were inoculated intracerebrally into transgenic (Tg) mice expressing MoPrP(89-231). The mice developed neurologic dysfunction between 380 and 660 days after inoculation. Brain extracts showed protease-resistant PrP by Western blotting; these extracts transmitted disease to wild-type FVB mice and Tg mice overexpressing PrP, with incubation times of 150 and 90 days, respectively. Neuropathological findings suggest that a novel prion strain was created. Our results provide compelling evidence that prions are infectious proteins. |
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